Troponin is the inhibitory or contractile regulating protein complex of striated muscle. It is located periodically along the thin filament of the muscle and consists of three distinct proteins: troponin I, troponin C, and troponin T. Measurement of troponin I values are useful in the evaluation of acute myocardial infarction (AMI). The cTnI ELISA is intended for the quantitative determination of cardiac troponin I in human serum.